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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA808748 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA808748, RRID:AB_2628971
- Product name
- LGR5 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)
- Antibody type
- Monoclonal
- Description
- LGR5 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LGR5
- Isotype
- IgG
- Antibody clone number
- OTI3F1
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.(1:2000)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of the stable expression of LGR5 (RC212825) in NIH3T3 cells using anti-LGR5 antibody (TA808748) (Red) compared to a nonspecific negative control antibody (Black).(1:100), surface stained on live cells
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of living HEK293 cells transfected with human LGR4,LGR5,LGR6 v1 and v2,mouse LGR4,LGR5 or LGR6 using anti-LGR5 antibody(TA808748)(1:50)
- Validation comment
- FC
