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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- UM800104 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM800104, RRID:AB_2629213
- Product name
- LGR5 mouse monoclonal antibody,clone UMAB212
- Antibody type
- Monoclonal
- Description
- LGR5 mouse monoclonal antibody,clone UMAB212
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LGR5
- Isotype
- IgG
- Antibody clone number
- UMAB212
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody(left lane: Brain right lane: Testis).(1:2000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- NIH-3T3 (Left lane) or stable expressed LGR5-3T3 (RC212825, Right lane) cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5.(1:1000)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody(1:2000). (Lanes: control vector pCMV6-Entry (PS100001); human LGR4 (RC221345), LGR5 (RC212825) and LGR6 (RC219284); or mouse LGR4(MR219497),LGR5 (MR219702) and LGR6(MR224646)).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LGR5 mouse monoclonal antibody (UM800104)immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY human LGR5(RC212825).(1:100)
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LGR5 mouse monoclonal antibody (UM800104) immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5(MR219702).(1:100)
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110C for 10 min, UM800104)(1:150)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human small intestine using anti-LGR5 clone UMAB212 mouse monoclonal antibody used at 1ug/mL dilution and detection with Polink2 Broad HRP DAB. UM800104 requires heat-induced epitope retrieval with TEE pH 9.0 at 95-100C 20 minutes. The image shows two arrows pointing to strong and weak staining of small intestine stem cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human gastric cancer using mouse anti-LGR5 clone UMAB212 (UM800104) at 1:400 with GBI Polink2 Broad HRP DAB detection kit (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 (B21-100) using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 clone UMAB212 (UM800104) at 1:400 with GBI Polink2 Broad HRP DAB detection kit (D22-110); pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 (B21-100)using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HEK293T cells transiently transfected with human LGR4 (RC221345) or mouse LGR4 (MR219497), human LGR5 (RC212825) or mouse LGR5 (MR219702), human LGR6 (RC219284) or mouse LGR6 (MR224646), or control vector pCMV6-Entry (PS100001), using anti-LGR5 antibody(UM800104, 1:100) surface stained on live cells.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of stable expression LGR5(RC212825) HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody(UM800104, 1:100) surface stained on live cells.
- Validation comment
- FC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-LGR5 mouse monoclonal antibody (UM800104) at 1:100. The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
- Validation comment
- 10K-CHIP
