Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MAB8078 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Lgr5/GPR49 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Lgr5/GPR49 in ELISA. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O75473
- Isotype
- IgG
- Antibody clone number
- 707042
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells.
Chiou GY, Yang TW, Huang CC, Tang CY, Yen JY, Tsai MC, Chen HY, Fadhilah N, Lin CC, Jong YJ
Scientific reports 2017 May 19;7(1):2172
Scientific reports 2017 May 19;7(1):2172
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with GFP (green) tagged mouse LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lgr5/GPR49 in NSO Mouse Cell Line Transfected with Human Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed NSO mouse myeloma cell line transfected with GFP (green) tagged human LGR5 using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lgr5/GPR49 in Mouse Cells Transfected with Mouse Lgr5/GPR49. Lgr5/GPR49 was detected in immersion fixed MYC tagged mouse transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm. Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lgr5/GPR49 in Human Cells Transfected with Human Lgr5/GPR49. Lgr5/GPR49 was detected in MYC tagged human transfectants using Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078). Cells were stained using an anti-mouse HRP-conjugated secondary antibody (brown). Specific staining was localized to cytoplasm. Image courtesy of Dr. Hans Clevers, Hubrecht Institute, The Netherlands.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with and either (A) Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Lgr5/GPR49 Antibody Induces Activity. Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB8078) induces TOPflash activity in the HEK293 human embryonic kidney cell line stably expressing LGR5 in the presence of Wnt-3a, but in the absence of R-Spondins. (Data courtesy of Dr. Wim de Lau and Dr. Hans Clevers, Hubrecht Institute, The Netherlands. See Reference 1.)