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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB8078P - Provider product page
- Provider
- R&D Systems
- Product name
- Human Lgr5/GPR49 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Lgr5/GPR49 in ELISAs. Stains human and mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry and Immunocytochemistry. This antibody, also known as "RD42", has been found to detect an epitope in the C-terminal LRR cap of Lgr5 (Ref. 1).
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
O75473- Isotype
- IgG
- Antibody clone number
- 707042
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Behavior of leucine-rich repeat-containing G-protein coupled receptor 5-expressing cells in the reprogramming process.
Arioka Y, Ito H, Hirata A, Semi K, Yamada Y, Seishima M
Stem cell research 2017 Apr;20:1-9
Stem cell research 2017 Apr;20:1-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human Lgr5/GPR49 and eGFP was stained with either (A) Mouse Anti-Human Lgr5/GPR49 PE-conjugated Monoclonal Antibody (Catalog # FAB8078P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
