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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Flow cytometry [1]
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- Product number
- ARG55261 - Provider product page
- Provider
- Arigo
- Product name
- anti-LGR5 / GPR49 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 689-719 of Human LGR5 (Swiss: O75473).
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) HeLa, 2) HepG2, 3) SH-SY5Y cell lysates stained with ARG55261 anti-LGR5 / GPR49 antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) HeLa whole cell lysates, 2) HepG2 whole cell lysates, 3) mouse skeletal muscle lysates, 4) SH-SY5Y whole cell lysates stained with ARG55261 anti-LGR5 / GPR49 antibody at 1:2000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) mouse skeletal muscle lysates, 2) rat skeletal muscle lysates, 3) Hela whole cell lysates, 4) HepG2 whole cell lysates, 5) SH-SY5Y whole cell lysates stained with XXXXX at 1:2000 dilution. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2 cell line lysates stained with ARG55261 anti-LGR5 / GPR49 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of mouse cerebellum tissue lysates stained with ARG55261 anti-LGR5 / GPR49 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HepG2 cells stained with ARG55261 anti-LGR5 / GPR49 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
