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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- F48168 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LGR-5 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 689-719 from the human protein was used as the immunogen for this LGR-5 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the LGR-5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of LGR-5 antibody at 1:2000 dilution. Lane 1: mouse skeletal muscle; 2: rat skeletal muscle; 3: HeLa; 4: HepG2; 5: SH-SY5Y lysate; Predicted band size : 100 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of LGR-5 antibody at 1:2000 dilution. Lane 1: HeLa lysate; 2: HepG2; 3: mouse cerebellum; 4: mouse skeletal muscle lysate; 5: SH-SY5Y; Predicted band size : 100 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cells stained with LGR-5 antibody at 1:100. Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm.
