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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AM26788PU-N - Provider product page
- Provider
- OriGene
- Product name
- Rat monoclonal anti-LGR5 / GPR49 antibody, cloneBEE-6F7, Purified
- Antibody type
- Monoclonal
- Description
- Rat monoclonal anti-LGR5 / GPR49 antibody, cloneBEE-6F7, Purified
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Clone BEE-6F7
- Vial size
- 0.1mg
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CGE analysis of purified AM26788PU-N?monoclonal antibody. Lane 1: molecularweight marker, Lane 2: 2 ug of purified AM26788PU-N?antibody. Proteins were separatedby CGE (capillary gel electrophoresis, Agilent 2100 Bioanalyzer). Internal controlbands (240 kDa / 7 kDa / 4,5 kDa).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using BEE-6F7. BOSC23 cells were transiently transfected with an expression vector encoding either LGR5-hum (green curve), or an irrelevant protein (control transfectant (red curve). Binding of BEE-6F7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with LGR5-hum transfected cells.
- Validation comment
- FC
