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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- TA400007 - Provider product page
- Provider
- OriGene
- Product name
- Anti-LGR5 mouse mAb, clone OTI2A2, bionylated
- Antibody type
- Monoclonal
- Description
- Anti-LGR5 mouse mAb, clone OTI2A2, bionylated
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LGR5
- Isotype
- IgG
- Antibody clone number
- OTI2A2
- Vial size
- 50 µg
- Concentration
- 0.73 mg/ml
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Supportive validation
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- OriGene (provider)
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- Experimental details
- HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY LGR5 (Lane 2, 3) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5-Biotin and Streptavidin-HRP (Sample was either boiled [lane 2] or not boiled [lane3] before loading to the gel).
- Validation comment
- WB
