CF503316

antibody from Invitrogen Antibodies

Targeting: LGR5 FEX, GPR49, GPR67, HG38

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

CF503316

Provider

Invitrogen Antibodies

Product name

LGR5 Monoclonal Antibody (OTI2A2), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2A2

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5. Positive lysates LY401213 (100 µg) and LC401213 (20 µg) can be purchased separately from OriGene.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-LGR5 mouse monoclonal antibody (TA503316) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-LGR5 mouse monoclonal antibody (TA503316) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5(RC212825).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA503316, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100 View Citation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: [Nature] cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. View CitationPubmed id: 27398792Memo: Human; IF

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified (A) and 400× magnified (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color. View Citation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200 View Citation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Positive correlation between LSD1, c-Myc, beta-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, beta-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either RC212825 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGR5 antibody (TA503316), and then analyzed by flow cytometry.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Cytofluorometric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values (6 SD) of consecutive experiments. Dilution: 1:400 View Citation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure from citation: Flow cytometry of SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of P2 population. Black: staining with Lgr5; red: staining with the isotype control. View Citation

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation(IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.