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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NBP1-28904 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28904, RRID:AB_1968504
- Product name
- Rabbit Polyclonal Lgr5/GPR49 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Robotically handled whole-tissue culture system for the screening of oral drug formulations.
Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice.
JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Leucine-rich repeat-containing G-protein-coupled receptor 5-positive cells in the endometrial stem cell niche.
Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis.
LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal.
von Erlach T, Saxton S, Shi Y, Minahan D, Reker D, Javid F, Lee YL, Schoellhammer C, Esfandiary T, Cleveland C, Booth L, Lin J, Levy H, Blackburn S, Hayward A, Langer R, Traverso G
Nature biomedical engineering 2020 May;4(5):544-559
Nature biomedical engineering 2020 May;4(5):544-559
Impact of a high‑fat diet on intestinal stem cells and epithelial barrier function in middle‑aged female mice.
Xie Y, Ding F, Di W, Lv Y, Xia F, Sheng Y, Yu J, Ding G
Molecular medicine reports 2020 Mar;21(3):1133-1144
Molecular medicine reports 2020 Mar;21(3):1133-1144
JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Kim MS, Cho HI, Yoon HJ, Ahn YH, Park EJ, Jin YH, Jang YK
Scientific reports 2018 Apr 26;8(1):6611
Scientific reports 2018 Apr 26;8(1):6611
Leucine-rich repeat-containing G-protein-coupled receptor 5-positive cells in the endometrial stem cell niche.
Cervelló I, Gil-Sanchis C, Santamaría X, Faus A, Vallvé-Juanico J, Díaz-Gimeno P, Genolet O, Pellicer A, Simón C
Fertility and sterility 2017 Feb;107(2):510-519.e3
Fertility and sterility 2017 Feb;107(2):510-519.e3
Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis.
Jung K, Saif LJ
Veterinary journal (London, England : 1997) 2015 May;204(2):134-43
Veterinary journal (London, England : 1997) 2015 May;204(2):134-43
LGR5 Activates Noncanonical Wnt Signaling and Inhibits Aldosterone Production in the Human Adrenal.
Shaikh LH, Zhou J, Teo AE, Garg S, Neogi SG, Figg N, Yeo GS, Yu H, Maguire JJ, Zhao W, Bennett MR, Azizan EA, Davenport AP, McKenzie G, Brown MJ
The Journal of clinical endocrinology and metabolism 2015 Jun;100(6):E836-44
The Journal of clinical endocrinology and metabolism 2015 Jun;100(6):E836-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Lgr5/GPR49 Antibody [NBP1-28904] - Staining on pig small intestine. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Lgr5/GPR49 Antibody [NBP1-28904] - Analysis of human small intestine.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Lgr5/GPR49 Antibody [NBP1-28904] - An intracellular stain was performed on SH-SY5Y cells with Lgr5/GPR49 Antibody [13A9] NBP1-28904AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Lgr5/GPR49 Antibody [NBP1-28904] - An intracellular stain was performed on HepG2 cells with Lgr5/GPR49 Antibody NBP1-28904AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
