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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-27178 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-LGR5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-27178 targets LGR5 in WB applications and shows reactivity with Human samples. The PA5-27178 immunogen is synthetic peptide corresponding to a region within amino acids 781 and 874 of Human LGR5.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.25 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LGR5 using 30µg of A) HeLa (B) HeLa membrane fraction (C) HepG2 (D) HepG2 membrane fraction and E) SK-N-SH lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a LGR5 polyclonal antibody (Product # PA5-27178) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LGR5 using 50 µg mouse muscle extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a LGR5 polyclonal antibody (Product # PA5-27178) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LGR5 in paraffin-embedded human intestine tissue using a LGR5 polyclonal antibody (Product # PA5-27178) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of LGR5 in paraffin-embedded human gastric carcinoma using a LGR5 polyclonal antibody (Product # PA5-27178) at a dilution of 1:100.
