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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP12377PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP12377PU-N, RRID:AB_1769181
- Product name
- anti LGR5 / GPR49
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-480 amino acids from the center region of human LGR5/GPR49.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (Cat.#AP12377PU-N) in K562 cell line lysates (35ug/lane). LGR5/GPR49(arrow) was detected using the purified Pab (1:60 dilution).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-LGR5/GPR49 Antibody (Center) (Cat.#AP12377PU-N) in HepG2 cell line lysates (35ug/lane). This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody (Center) (Cat.#AP12377PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
