ABIN389218

antibody from antibodies-online

Targeting: LGR5 FEX, GPR49, GPR67, HG38

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN389218

Provider

antibodies-online

Product name

anti-Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5) (AA 689-719), (Loop 2) antibody

Antibody type

Polyclonal

Description

This antibody is purified through a protein A column, followed by peptide affinity purification.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

AA 689-719, Loop 2

Antibody clone number

RB14211

Vial size

400 μL

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots.

Handling

Avoid freeze-thaw cycles.

References

Submitted references

Long-term culture of genome-stable bipotent stem cells from adult human liver.

Huch M, Gehart H, van Boxtel R, Hamer K, Blokzijl F, Verstegen MM, Ellis E, van Wenum M, Fuchs SA, de Ligt J, van de Wetering M, Sasaki N, Boers SJ, Kemperman H, de Jonge J, Ijzermans JN, Nieuwenhuis EE, Hoekstra R, Strom S, Vries RR, van der Laan LJ, Cuppen E, Clevers H
Cell 2015 Jan 15;160(1-2):299-312

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LGR5 is a promising biomarker for patients with stage I and II gastric cancer.

Bu Z, Zheng Z, Zhang L, Li Z, Sun Y, Dong B, Wu A, Wu X, Wang X, Cheng X, Xing X, Li Y, Du H, Ji J
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2013 Feb;25(1):79-89

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Intestinal stem cell marker LGR5 expression during gastric carcinogenesis.

Zheng ZX, Sun Y, Bu ZD, Zhang LH, Li ZY, Wu AW, Wu XJ, Wang XH, Cheng XJ, Xing XF, Du H, Ji JF
World journal of gastroenterology 2013 Dec 14;19(46):8714-21

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Transcription factor Oct1 is a somatic and cancer stem cell determinant.

Maddox J, Shakya A, South S, Shelton D, Andersen JN, Chidester S, Kang J, Gligorich KM, Jones DA, Spangrude GJ, Welm BE, Tantin D
PLoS genetics 2012;8(11):e1003048

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Significance of Lgr5(+ve) cancer stem cells in the colon and rectum.

Takahashi H, Ishii H, Nishida N, Takemasa I, Mizushima T, Ikeda M, Yokobori T, Mimori K, Yamamoto H, Sekimoto M, Doki Y, Mori M
Annals of surgical oncology 2011 Apr;18(4):1166-74

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Stable expression of neurogenin 1 induces LGR5, a novel stem cell marker, in an immortalized human neural stem cell line HB1.F3.

Satoh J, Obayashi S, Tabunoki H, Wakana T, Kim SU
Cellular and molecular neurobiology 2010 Apr;30(3):415-26

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Identification of stem cells in small intestine and colon by marker gene Lgr5.

Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H
Nature 2007 Oct 25;449(7165):1003-7

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

WB

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

IHC

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS