PA5-120636

antibody from Invitrogen Antibodies

Targeting: GTF3C3 TFIIIC102, TFiiiC2-102

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120636

Provider

Invitrogen Antibodies

Product name

GTF3C3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HepG2, HeLa, 293T, RAW264.7, NIH/3T3, Mouse lung. Immunogen sequence: MSGFSPELID YLEGKISFEE FERRREERKT REKKSLQEKG KLSAEENPDD SEVPSSSGIN STKSQDKDVN EGETSDGVRK SVHKVFASML GENEDDEEEE EEEEEEEEEE ETPEQPTAGD VFVLEMVLNR ETKKMMKEKR PRSKLPRALR GLMGEANIRF

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.98 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GTF3C3 in extracts of various cell lines. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GTF3C3 in H9C2 cells. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of GTF3C3 in H9C2 cells using a GTF3C3 Polyclonal antibody (Product # PA5-120636). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GTF3C3 in U2OS cells. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GTF3C3 in paraffin-embedded rat brain. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GTF3C3 in paraffin-embedded mouse brain. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GTF3C3 in paraffin-embedded rat brain. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GTF3C3 in paraffin-embedded mouse brain. Samples were incubated with GTF3C3 Polyclonal antibody (Product # PA5-120636) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.