HPA011889

antibody from Atlas Antibodies

Targeting: C1orf116 FLJ36507, MGC2742, MGC4309, SARG

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA011889

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA011889, RRID:AB_1853873

Product name

Anti-C1orf116

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SGEDPNSRLAPLTTPKPRKLPPNIVLKSSRSSFHS
DPQHWLSRHTEAAPGDSGLISCSLQEQRKARKEAL
EKLGLPQDQDEPGLHLSKPTSSIRPKETRAQHLSP
APGLAQPAAPAQASAAIPAAG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-C1orf116 antibody HPA011889 (A) shows similar pattern to independent antibody HPA011888 (B).

Antibody #2 product nr

HPA011888

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Esophagus tissue

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human stomach and liver tissues using Anti-C1orf116 antibody. Corresponding C1orf116 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN