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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA048813 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA048813, RRID:AB_2680529
- Product name
- Anti-BOLL
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human BOLL, Gene description: boule-like RNA-binding protein, Alternative Gene Names: BOULE, Validated applications: WB, IHC, Uniprot ID: Q8N9W6, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.5 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Haploid Germ Cells Generated in Organotypic Culture of Testicular Tissue From Prepubertal Boys
de Michele F, Poels J, Vermeulen M, Ambroise J, Gruson D, Guiot Y, Wyns C
Frontiers in Physiology 2018;9
Frontiers in Physiology 2018;9
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and BOLL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409790).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and kidney tissues using Anti-BOLL antibody. Corresponding BOLL RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
