HPA048813

antibody from Atlas Antibodies

Targeting: BOLL BOULE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA048813

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA048813, RRID:AB_2680529

Product name

Anti-BOLL

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQ
QVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYF
HTPEVTSVPPPWPSRSVCSSPVMVAQPIYQ


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and BOLL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409790).

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and kidney tissues using Anti-BOLL antibody. Corresponding BOLL RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human colon, liver, lymph node and testis using Anti-BOLL antibody HPA048813 (A) shows similar protein distribution across tissues to independent antibody HPA018678 (B).

Antibody #2 product nr

HPA018678

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver using Anti-BOLL antibody HPA048813.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-BOLL antibody HPA048813.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-BOLL antibody HPA048813.

Sample type

HUMAN