Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [9]
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Validation data
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- Product number
- NBP1-83619 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-83619, RRID:AB_11014892
- Product name
- Rabbit Polyclonal SATB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human SATB2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Generation of monospecific antibodies based on affinity capture of polyclonal antibodies.
SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas.
Extensive expression of craniofacial related homeobox genes in canine mammary sarcomas.
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Hjelm B, Forsström B, Igel U, Johannesson H, Stadler C, Lundberg E, Ponten F, Sjöberg A, Rockberg J, Schwenk JM, Nilsson P, Johansson C, Uhlén M
Protein science : a publication of the Protein Society 2011 Nov;20(11):1824-35
Protein science : a publication of the Protein Society 2011 Nov;20(11):1824-35
SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinomas.
Magnusson K, de Wit M, Brennan DJ, Johnson LB, McGee SF, Lundberg E, Naicker K, Klinger R, Kampf C, Asplund A, Wester K, Gry M, Bjartell A, Gallagher WM, Rexhepaj E, Kilpinen S, Kallioniemi OP, Belt E, Goos J, Meijer G, Birgisson H, Glimelius B, Borrebaeck CA, Navani S, Uhlén M, O'Connor DP, Jirström K, Pontén F
The American journal of surgical pathology 2011 Jul;35(7):937-48
The American journal of surgical pathology 2011 Jul;35(7):937-48
Extensive expression of craniofacial related homeobox genes in canine mammary sarcomas.
Wensman H, Göransson H, Leuchowius KJ, Strömberg S, Pontén F, Isaksson A, Rutteman GR, Heldin NE, Pejler G, Hellmén E
Breast cancer research and treatment 2009 Nov;118(2):333-43
Breast cancer research and treatment 2009 Nov;118(2):333-43
From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Ek S, Andréasson U, Hober S, Kampf C, Pontén F, Uhlén M, Merz H, Borrebaeck CA
Molecular & cellular proteomics : MCP 2006 Jun;5(6):1072-81
Molecular & cellular proteomics : MCP 2006 Jun;5(6):1072-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SATB2 Antibody [NBP1-83619] - Simple Western lane view shows a specific band for SATB2 in 0.1 mg/ml of THP1 (left), HDLM2 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SATB2 Antibody [NBP1-83619] - Electropherogram image(s) of corresponding Simple Western lane view. SATB2 antibody was used at 1:50 dilution on THP1 and HDLM2 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SATB2 Antibody [NBP1-83619] - Analysis in human cell line HELA.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Immunohistochemical staining of human liver shows no positivity as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human rectum.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Analysis in human colon and liver tissues. Corresponding SATB2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human colon shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human cerebral cortex, colon, liver and rectum using Anti-SATB2 antibody NBP1-83619 (A) shows similar protein distribution across tissues to independent antibody NBP1-92365 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-83619] - Staining of human rectum shows strong nuclear positivity in glandular cells.