LS-C154229

antibody from LSBio

Targeting: CYTIP B3-1, CASP, CYBR, CYTHIP, HE, PSCDBP

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Antibody Data

Product number

LS-C154229

Provider

LSBio

Product name

CYTIP Antibody LS-C154229

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Cybr Antibody - Western Blot. Western blot of affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct Western blot of wt splenocytes. Lane 6 shows direct Western blot of knock out mouse. Personal Communication, V. Coppola, NCI, Bethesda, MD.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Cybr Antibody - Western Blot. Western blot of affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct Western blot of wt splenocytes. Lane 6 shows direct Western blot of knock out mouse. Personal Communication, V. Coppola, NCI, Bethesda, MD.