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LearnNBP2-22477
antibody from Novus Biologicals
Targeting: SYNE1
8B, ARCA1, C6orf98, CPG2, dJ45H2.2, enaptin, KIAA0796, MYNE1, Nesp1, Nesprin-1, SCAR8, SYNE-1B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- NBP2-22477 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal SYNE1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SYNE1 Antibody (6A) [NBP2-22477] - Analysis of CPG2 is shown using adult rat brain fractions and whole cell lysates from mock or CPG2 transfected HEK293T cells (kindly provided by Elly Nedivi's lab, MIT, Cambridge, MA) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CPG2 at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura.
