H00008837-D01P

antibody from Abnova Corporation

Targeting: CFLAR c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008837-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008837-D01P, RRID:AB_1573043

Product name

CFLAR purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human CFLAR protein.

Antigen sequence

MSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPP
NVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKR
ILKMDRKAVETHLLRNPHLVSDYRVLMAEIGEDLD
KSDVSSLIFLMKDYMGRGKISKEKSFLDLVVELEK
LNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQSV
QGAGTSYRNVLQAAIQKSLKDPSNNFRLHNGRSKE
QRLKEQLGAQQEPVKKSIQESEAFLPQSIPEERYK
MKSKPLGICLIIDCIGNETELLRDTFTSLGYEVQK
FLHLSMHGISQILGQFACMPEHRDYDSFVCVLVSR
GGSQSVYGVDQTHSGLPLHHIRRMFMGDSCPYLAG
KPKMFFIQNYVVSEGQLEDSSLLEVDGPAMKNVEF
KAQKRGLCTVHREADFFWSLCTADMSLLEQSHSSP
SLYLQCLSQKLRQERKRPLLDLHIELNGYMYDWNS
RVSAKEKYYVWLQHTLRKKLILSYT


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CFLAR expression in transfected 293T cell line (H00008837-T03) by CFLAR MaxPab polyclonal antibody.Lane 1: CFLAR transfected lysate(55.30 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between CFLAR and CASP10. HeLa cells were stained with anti-CFLAR rabbit purified polyclonal 1:1200 and anti-CASP10 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)