AP21805SU-S

antibody from Acris Antibodies GmbH

Targeting: CFLAR c-FLIP, CASH, CASP8AP1, Casper, cFLIP, CLARP, FLAME, FLIP, I-FLICE, MRIT

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP21805SU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP21805SU-S, RRID:AB_10756329

Product name

anti CFLAR / Casper / I-FLICE (Long and Short)

Antibody type

Polyclonal

Antigen

A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP was

Reactivity

Human

Host

Rabbit

Vial size

50 µl

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using AP21805SU-S at 1/2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-eosin counterstain.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using AP21805SU-S at 1/2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.