16208-1-AP

antibody from Proteintech Group

Targeting: MTF2 M96, PCL2, TDRD19A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16208-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16208-1-AP, RRID:AB_2147370

Product name

MTF2 antibody

Antibody type

Polyclonal

Description

KD/KO validated MTF2 antibody (Cat. #16208-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Embryonic stem cell factors DPPA2/4 amplify active H3K4me3-H2AK119ub chromatin domains in non-small cell lung cancer.

Seneviratne JA, Crisp CL, Glancy E, Choy N, Tan W, Neve M, Stammers M, Wang T, Johnstone R, Kaur A, Fennell KA, Burr ML, Parker BL, Shakeel S, Eckersley-Maslin MA
Genes & development 2026 May 4;40(9-10):756-776

---

Accessory subunits of PRC2 mimic H3K27me3 to restrict the spread of Polycomb domains.

Agius SC, Boudes M, Healy E, Kim Y, Chang YC, Murray M, Manent J, Rientjes J, Lai Z, Nixon S, Homman-Ludiye J, Levina V, McGlinn E, Davidovich C
Molecular cell 2026 Mar 19;86(6):1032-1045.e10

---

EPOP restricts PRC2.1 targeting to chromatin by directly modulating enzyme complex dimerization.

Gong L, Liu X, Yang X, Yu Z, Bhat MY, Chen S, Lemoff A, Xing C, Liu X
Nature communications 2026 Jan 10;17(1):1565

---

Unbalanced chromatin binding of Polycomb complexes drives neurodevelopmental disorders.

Borges RL, González-Blanco G, Arigela H, Huang Y, Caeiro LD, Fattakhov N, Lepore S, Garcia-Martinez L, Maurice M, Mehta PD, Park EJ, MacGillivray K, Nehru J, Chau M, Robayo MC, Abad C, Bilbao-Martinez A, Monteiro F, Luo X, Tan S, Bilbao D, Sidoli S, Di Stefano B, Walz K, Saltzman AL, Verdun RE, Shiekhattar R, Morey L
Molecular cell 2026 Feb 19;86(4):604-624.e16

---

Macromolecular interactions dictate Polycomb-mediated epigenetic repression.

Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
bioRxiv : the preprint server for biology 2025 May 15;

---

Isogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAs.

Trotman JB, Abrash EW, Murvin MM, Braceros AK, Li S, Boyson SP, Salcido RT, Cherney RE, Bischoff SR, Kaufmann K, Eberhard QE, Zhang Z, Cowley DO, Calabrese JM
Molecular cell 2025 Mar 20;85(6):1117-1133.e14

---

Distinct specificity and functions of PRC2 subcomplexes in human stem cells and cardiac differentiation.

Much C, Rajkumar SM, Chen L, Cohen JM, Gade AR, Pitt GS, Long Y
Molecular cell 2025 Aug 21;85(16):3057-3073.e10

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PCIF1 drives oesophageal squamous cell carcinoma progression via m6Am-mediated suppression of MTF2 translation.

Li K, Yi Y, Ling R, Zhang C, Zhang Z, Wang Y, Wang G, Chen J, Cheng M, Chen S
Clinical and translational medicine 2025 Apr;15(4):e70286

---

EPOP Restricts PRC2.1 Targeting to Chromatin by Directly Modulating Enzyme Complex Dimerization.

Gong L, Liu X, Yang X, Yu Z, Chen S, Xing C, Liu X
bioRxiv : the preprint server for biology 2024 Sep 10;

---

Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA Airn.

Braceros AK, Schertzer MD, Omer A, Trotman JB, Davis ES, Dowen JM, Phanstiel DH, Aiden EL, Calabrese JM
Cell reports 2023 Jul 25;42(7):112803

---

Combined inhibition of EZH2 and the autotaxin-LPA-LPA2 axis exerts synergistic antitumor effects on colon cancer cells.

Long Y, Wang Y, Qu M, Zhang D, Zhang X, Zhang J
Cancer letters 2023 Jul 10;566:216226

---

Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes.

Sparbier CE, Gillespie A, Gomez J, Kumari N, Motazedian A, Chan KL, Bell CC, Gilan O, Chan YC, Popp S, Gough DJ, Eckersley-Maslin MA, Dawson SJ, Lehner PJ, Sutherland KD, Ernst P, McGeehan GM, Lam EYN, Burr ML, Dawson MA
Nature cell biology 2023 Feb;25(2):258-272

---

Identification of the Transcription Co-Factor-Related Gene Signature and Risk Score Model for Osteosarcoma.

Jin Z, Wu J, Lin J, Wang J, Shen Y
Frontiers in genetics 2022;13:862803

---

JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation.

Tavares M, Khandelwal G, Muter J, Viiri K, Beltran M, Brosens JJ, Jenner RG
Cell reports 2022 May 31;39(9):110889

---

The transcriptional elongation factor CTR9 demarcates PRC2-mediated H3K27me3 domains by altering PRC2 subtype equilibrium.

Chan NT, Huang J, Ma G, Zeng H, Donahue K, Wang Y, Li L, Xu W
Nucleic acids research 2022 Feb 28;50(4):1969-1992

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PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing.

Fong KW, Zhao JC, Lu X, Kim J, Piunti A, Shilatifard A, Yu J
Molecular cell 2022 Dec 15;82(24):4611-4626.e7

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Polycomb Factor PHF19 Controls Cell Growth and Differentiation Toward Erythroid Pathway in Chronic Myeloid Leukemia Cells.

García-Montolio M, Ballaré C, Blanco E, Gutiérrez A, Aranda S, Gómez A, Kok CH, Yeung DT, Hughes TP, Vizán P, Di Croce L
Frontiers in cell and developmental biology 2021;9:655201

---

BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation.

Conway E, Rossi F, Fernandez-Perez D, Ponzo E, Ferrari KJ, Zanotti M, Manganaro D, Rodighiero S, Tamburri S, Pasini D
Molecular cell 2021 Sep 2;81(17):3526-3541.e8

---

Distinct PRC2 subunits regulate maintenance and establishment of Polycomb repression during differentiation.

Petracovici A, Bonasio R
Molecular cell 2021 Jun 17;81(12):2625-2639.e5

---

Competition between PRC2.1 and 2.2 subcomplexes regulates PRC2 chromatin occupancy in human stem cells.

Youmans DT, Gooding AR, Dowell RD, Cech TR
Molecular cell 2021 Feb 4;81(3):488-501.e9

---

RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells.

Long Y, Hwang T, Gooding AR, Goodrich KJ, Rinn JL, Cech TR
Nature genetics 2020 Sep;52(9):931-938

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Cell Signaling Coordinates Global PRC2 Recruitment and Developmental Gene Expression in Murine Embryonic Stem Cells.

Aljazi MB, Gao Y, Wu Y, Mias GI, He J
iScience 2020 Nov 20;23(11):101646

---

A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island Chromatin.

Chen S, Jiao L, Liu X, Yang X, Liu X
Molecular cell 2020 Mar 19;77(6):1265-1278.e7

---

PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells.

Jain P, Ballare C, Blanco E, Vizan P, Di Croce L
eLife 2020 Mar 10;9

---

Polycomb-like 2 regulates PRC2 components to affect proliferation in glioma cells.

Wang F, Gao Y, Lv Y, Wu Y, Guo Y, Du F, Wang S, Yu J, Cao X, Li PA
Journal of neuro-oncology 2020 Jun;148(2):259-271

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Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression.

Tamburri S, Lavarone E, Fernández-Pérez D, Conway E, Zanotti M, Manganaro D, Pasini D
Molecular cell 2020 Feb 20;77(4):840-856.e5

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G-tract RNA removes Polycomb repressive complex 2 from genes.

Beltran M, Tavares M, Justin N, Khandelwal G, Ambrose J, Foster BM, Worlock KB, Tvardovskiy A, Kunzelmann S, Herrero J, Bartke T, Gamblin SJ, Wilson JR, Jenner RG
Nature structural & molecular biology 2019 Oct;26(10):899-909

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PRC2.1 and PRC2.2 Synergize to Coordinate H3K27 Trimethylation.

Healy E, Mucha M, Glancy E, Fitzpatrick DJ, Conway E, Neikes HK, Monger C, Van Mierlo G, Baltissen MP, Koseki Y, Vermeulen M, Koseki H, Bracken AP
Molecular cell 2019 Nov 7;76(3):437-452.e6

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Dissecting the role of H3K27 acetylation and methylation in PRC2 mediated control of cellular identity.

Lavarone E, Barbieri CM, Pasini D
Nature communications 2019 Apr 11;10(1):1679

---

A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.

Conway E, Jerman E, Healy E, Ito S, Holoch D, Oliviero G, Deevy O, Glancy E, Fitzpatrick DJ, Mucha M, Watson A, Rice AM, Chammas P, Huang C, Pratt-Kelly I, Koseki Y, Nakayama M, Ishikura T, Streubel G, Wynne K, Hokamp K, McLysaght A, Ciferri C, Di Croce L, Cagney G, Margueron R, Koseki H, Bracken AP
Molecular cell 2018 May 3;70(3):408-421.e8

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Capturing the Onset of PRC2-Mediated Repressive Domain Formation.

Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D
Molecular cell 2018 Jun 21;70(6):1149-1162.e5

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MTF2 recruits Polycomb Repressive Complex 2 by helical-shape-selective DNA binding.

Perino M, van Mierlo G, Karemaker ID, van Genesen S, Vermeulen M, Marks H, van Heeringen SJ, Veenstra GJC
Nature genetics 2018 Jul;50(7):1002-1010

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Polycomb-like proteins link the PRC2 complex to CpG islands.

Li H, Liefke R, Jiang J, Kurland JV, Tian W, Deng P, Zhang W, He Q, Patel DJ, Bulyk ML, Shi Y, Wang Z
Nature 2017 Sep 14;549(7671):287-291

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Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.

Hunkapiller J, Shen Y, Diaz A, Cagney G, McCleary D, Ramalho-Santos M, Krogan N, Ren B, Song JS, Reiter JF
PLoS genetics 2012;8(3):e1002576

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 16208-1-AP(MTF2 antibody) at dilution of 1:600

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16208-1-AP(MTF2 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16208-1-AP(MTF2 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue