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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-33559 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FZD7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Porcine, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Frizzled-7 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FZD7 Polyclonal Antibody (Product # PA5-33559) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Blue) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma Membrane, Endoplasmic Reticulum and Cytoplasmic vesicular localization. Panel e represents SK-BR-3 cells having no expression of FZD7. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FZD7 in Human Colon. Samples were incubated with FZD7 polyclonal antibody (Product # PA5-33559). formalin-fixed paraffin-embedded .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FZD7 in Human Breast carcinoma. Samples were incubated with FZD7 polyclonal antibody (Product # PA5-33559). formalin-fixed, paraffin-embedded.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FZD7 in Human Prostate Carcinoma. Samples were incubated with FZD7 polyclonal antibody (Product # PA5-33559). formalin-fixed paraffin-embedded .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FZD7 in Human Prostate. Samples were incubated with FZD7 polyclonal antibody (Product # PA5-33559). formalin-fixed paraffin-embedded .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FZD7 in Human Small Intestine. Samples were incubated with FZD7 polyclonal antibody (Product # PA5-33559). formalin-fixed paraffin-embedded .
