Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB1981-050 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Frizzled-7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q61090
- Isotype
- IgG
- Antibody clone number
- 151143
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.
Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
Wu DT, Seita Y, Zhang X, Lu CW, Roth MJ
PloS one 2012;7(4):e34778
PloS one 2012;7(4):e34778
Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
Vijayaragavan K, Szabo E, Bossé M, Ramos-Mejia V, Moon RT, Bhatia M
Cell stem cell 2009 Mar 6;4(3):248-62
Cell stem cell 2009 Mar 6;4(3):248-62
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Frizzled-7 in HEK293 Human Cell Line. Frizzled-7 was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human/Mouse Frizzled-7 Monoclonal Antibody (Catalog # MAB1981) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI(blue). Specific staining was localized to the cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.