PAB21352

antibody from Abnova Corporation

Targeting: NOP58 HSPC120, NOP5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21352

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21352, RRID:AB_10967687

Product name

NOP5/NOP58 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NOP5/NOP58.

Antigen sequence

LDKELNNYIMRCREWYGWHFPELGKIISDNLTYCK
CLQKVGDRKNYASAKLSELLPEEVEAEVKAAAEIS
MGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQ
NRMMAIAPNVTVMVGELVGARLIAHAGSLL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with NOP5/NOP58 polyclonal antibody (Cat # PAB21352) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NOP5/NOP58 polyclonal antibody (Cat # PAB21352) at 1-4 ug/mL dilution shows positivity in nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with NOP5/NOP58 polyclonal antibody (Cat # PAB21352) shows nuclear and cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)