Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-54321 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOP58 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LDKELNNYIM RCREWYGWHF PELGKIISDN LTYCKCLQKV GDRKNYASAK LSELLPEEVE AEVKAAAEIS MGTEVSEEDI CNILHLCTQV IEISEYRTQL YEYLQNRMMA IAPNVTVMVG ELVGARLIAH AGSLL Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP58 in mouse cell line NIH-3T3 and rat cell line NBT-II using a NOP58 Polyclonal Antibody (Product # PA5-54321).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP58 using NOP58 Polyclonal Antibody (Product # PA5-54321) (A) shows similar pattern to an independent NOP58 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NOP58 in human cell line U-2 OS shows positivity in nucleoli. Samples were probed using a NOP58 Polyclonal Antibody (Product # PA5-54321).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of NOP58 in human cell line U-251 MG using a NOP58 Polyclonal Antibody (Product # PA5-54321) shows localization to nucleoli fibrillar center.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of NOP58 in human stomach tissue shows nuclear and cytoplasmic positivity in glandular cells. Samples were probed using a NOP58 Polyclonal Antibody (Product # PA5-54321).