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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-30708 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOP58 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (80%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOP58 using 30 µg of Jurkat lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a NOP58 polyclonal antibody (Product # PA5-30708) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NOP58 Polyclonal Antibody (Product # PA5-30708). Sample (50 µg of whole cell lysate). Lane A: mouse brain . 7.5% SDS PAGE. NOP58 Polyclonal Antibody (Product # PA5-30708) diluted at 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NOP58 Polyclonal Antibody (Product # PA5-30708). Jurkat whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NOP58 Polyclonal Antibody (Product # PA5-30708) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
