PA5-112219

antibody from Invitrogen Antibodies

Targeting: WDR12 FLJ10881, YTM1

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112219

Provider

Invitrogen Antibodies

Product name

WDR12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WDR12 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate, Lane B: 0.5 mg HeLa Whole Cell Lysate, Lane C: 0.5 mg 293T Whole Cell Lysate, Lane D: 0.5 mg NIH3T3 Whole Cell Lysate 4 µL with WDR12 Polyclonal Antibody (Product # PA5-112219) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: WDR12 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 48 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of WDR12 was performed on (Lane A) 0.5 mg 293T whole cell lysate using 4 µL of WDR12 Polyclonal Antibody (Product # PA5-112219) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Clean-Blot IP Detection Reagent (HRP) was used as a secondary antibody at a dilution of 1:1,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 48 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WDR12 Immunoprecipitation using: Lane A: 0.5 mg A431 Whole Cell Lysate, Lane B: 0.5 mg HeLa Whole Cell Lysate, Lane C: 0.5 mg 293T Whole Cell Lysate, Lane D: 0.5 mg NIH3T3 Whole Cell Lysate 4 µL with WDR12 Polyclonal Antibody (Product # PA5-112219) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: WDR12 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 48 kDa.