25750-1-AP

antibody from Invitrogen Antibodies

Targeting: RAPH1 ALS2CR18, ALS2CR9, KIAA1681

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25750-1-AP

Provider

Invitrogen Antibodies

Product name

RAPH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEQLSDEEI DHGAEEDSDK EDQDLDKMFG AWLGELDKLT QSLDSDKPME PVKRSPLRQE TNMANFSYRF SIYNLNEALN QGETVDLDAL MADLCSIEQE LSSIGSGNSK RQITETKATQ KLPVSRHTLK HGTLKGLSSS SNRIAKPSHA SYSLDDVTAQ LEQASLSMDE AAQQSVLEDT KPLVTNQHRR TASAGTVSDA EVHSISNSSH SSITSAASSM DSLDIDKVTR PQELDLTHQG QPIT (1-243 aa encoded by BC156922)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25750-1-AP ( RAPH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 25750-1-AP (RAPH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 25750-1-AP (RAPH1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 25750-1-AP (RAPH1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 25750-1-AP ( RAPH1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tissue slide using 25750-1-AP ( RAPH1 Antibody) at dilution of 1:50 (under 40x lens).