Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-78547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTBP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A549, H1299, HCT116, Mouse eye Predicted reactivity: Mouse (99%), Rat (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTBP2 in mouse tissue extract using 50 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with PTBP2 polyclonal antibody (Product # PA5-78547) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTBP2 in A) A549 whole cell lysate, B) H1299 whole cell lysate, C) HCT116 whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with PTBP2 polyclonal antibody (Product # PA5-78547) using a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP2 Polyclonal Antibody detects PTBP2 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PTBP2 Polyclonal Antibody (Product # PA5-78547) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP2 Polyclonal Antibody detects PTBP2 protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract. B. 30 µg H1299 whole cell lysate/extract. C. 30 µg HCT116 whole cell lysate/extract.7.5 % SDS-PAGE. PTBP2 Polyclonal Antibody (Product # PA5-78547) dilution: 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of PTBP2 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PTBP2 Polyclonal Antibody (Product # PA5-78547) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP2 Polyclonal Antibody detects polypyrimidine tract binding protein 2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: polypyrimidine tract binding protein 2 protein stained by PTBP2 Polyclonal Antibody (Product # PA5-78547) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP2 Polyclonal Antibody detects PTBP2 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PTBP2 Polyclonal Antibody (Product # PA5-78547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP2 Polyclonal Antibody detects PTBP2 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. PTBP2 Polyclonal Antibody (Product # PA5-78547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PTBP2 Polyclonal Antibody detects PTBP2 protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. PTBP2 Polyclonal Antibody (Product # PA5-78547) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.