Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 302902 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#302902, RRID:AB_314304
- Product name
- Purified anti-human CD28
- Antibody type
- Monoclonal
- Antigen
- CD28
- Description
- CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references Association of major histocompatibility complex class I haplotypes with disease progression after simian immunodeficiency virus challenge in burmese rhesus macaques.
HIV-1 Nef disrupts intracellular trafficking of major histocompatibility complex class I, CD4, CD8, and CD28 by distinct pathways that share common elements.
Burkholderia pseudomallei infection of T cells leads to T-cell costimulation partially provided by flagellin.
Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities.
Ex vivo simulation of drug action: quantification of drug-induced mRNA as a bridge between preclinical and clinical trials.
A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes.
Nomura T, Yamamoto H, Shiino T, Takahashi N, Nakane T, Iwamoto N, Ishii H, Tsukamoto T, Kawada M, Matsuoka S, Takeda A, Terahara K, Tsunetsugu-Yokota Y, Iwata-Yoshikawa N, Hasegawa H, Sata T, Naruse TK, Kimura A, Matano T
Journal of virology 2012 Jun;86(12):6481-90
Journal of virology 2012 Jun;86(12):6481-90
HIV-1 Nef disrupts intracellular trafficking of major histocompatibility complex class I, CD4, CD8, and CD28 by distinct pathways that share common elements.
Leonard JA, Filzen T, Carter CC, Schaefer M, Collins KL
Journal of virology 2011 Jul;85(14):6867-81
Journal of virology 2011 Jul;85(14):6867-81
Burkholderia pseudomallei infection of T cells leads to T-cell costimulation partially provided by flagellin.
Ye Z, Lee CM, Sun GW, Gan YH
Infection and immunity 2008 Jun;76(6):2541-50
Infection and immunity 2008 Jun;76(6):2541-50
Human amnion mesenchyme harbors cells with allogeneic T-cell suppression and stimulation capabilities.
Magatti M, De Munari S, Vertua E, Gibelli L, Wengler GS, Parolini O
Stem cells (Dayton, Ohio) 2008 Jan;26(1):182-92
Stem cells (Dayton, Ohio) 2008 Jan;26(1):182-92
Ex vivo simulation of drug action: quantification of drug-induced mRNA as a bridge between preclinical and clinical trials.
Mitsuhashi M
Clinical chemistry 2007 Jan;53(1):148-9
Clinical chemistry 2007 Jan;53(1):148-9
A major role of PKC theta and NFkappaB in the regulation of hTERT in human T lymphocytes.
Sheng WY, Chen YR, Wang TC
FEBS letters 2006 Dec 22;580(30):6819-24
FEBS letters 2006 Dec 22;580(30):6819-24
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Whole blood lymphocytes stainedwith CD28.2 PE
- Conjugate
- Unconjugated