Ab185378

antibody from Aladdin Scientific

Targeting: CD28

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab185378

Provider

Aladdin Scientific

Product name

CD28 Mouse mAb (Biotin)

Antibody type

Monoclonal

Description

Flow: ²0.5 _g/testFor flow cytometric staining, the suggested use of this reagent is ²0.5 _g per million cells in 100 µL staining volume or 100 µL of whole blood.

Reactivity

Human

Host

Mouse

Conjugate

Biotin

Antibody clone number

CD28.2

Vial size

100_g,10_g,25_g

Concentration

0,5 mg/ml

Storage

The antibody solution should be stored undiluted between 2¡C and 8¡C, and protected from prolonged exposure to light. Do not freeze.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

CD62L Mouse mAb (Biotin) (Ab179520) - Flow Cytometry Flow cytometry analysis of CD28 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD28 Mouse mAb (Biotin) (Ab185378) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.