Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- AF-342-PB - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD28 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD28 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse CD28 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P10747
- Isotype
- IgG
- Vial size
- 200 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Diversification of Bw4 Specificity and Recognition of a Nonclassical MHC Class I Molecule Implicated in Maternal-Fetal Tolerance by Killer Cell Ig-like Receptors of the Rhesus Macaque.
Phenotype, function, and differentiation potential of human monocyte subsets.
Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus.
CD28-mediated regulation of multiple myeloma cell proliferation and survival.
The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
Banerjee P, Ries M, Janaka SK, Grandea AG 3rd, Wiseman R, O'Connor DH, Golos TG, Evans DT
Journal of immunology (Baltimore, Md. : 1950) 2018 Nov 1;201(9):2776-2786
Journal of immunology (Baltimore, Md. : 1950) 2018 Nov 1;201(9):2776-2786
Phenotype, function, and differentiation potential of human monocyte subsets.
Boyette LB, Macedo C, Hadi K, Elinoff BD, Walters JT, Ramaswami B, Chalasani G, Taboas JM, Lakkis FG, Metes DM
PloS one 2017;12(4):e0176460
PloS one 2017;12(4):e0176460
Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus.
Ellinghaus U, Cortini A, Pinder CL, Le Friec G, Kemper C, Vyse TJ
European journal of immunology 2017 Jul;47(7):1200-1210
European journal of immunology 2017 Jul;47(7):1200-1210
CD28-mediated regulation of multiple myeloma cell proliferation and survival.
Bahlis NJ, King AM, Kolonias D, Carlson LM, Liu HY, Hussein MA, Terebelo HR, Byrne GE Jr, Levine BL, Boise LH, Lee KP
Blood 2007 Jun 1;109(11):5002-10
Blood 2007 Jun 1;109(11):5002-10
The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
Sakthivel P, Shively V, Kakoulidou M, Pearce W, Lefvert AK
Journal of internal medicine 2007 Apr;261(4):399-407
Journal of internal medicine 2007 Apr;261(4):399-407
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD28 in Human PBMCs. CD28 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human CD28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-342-PB) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD28 in Human Tonsil. CD28 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human CD28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-342-PB) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human CD28 Antibody Enhances IL-2 Secretion in Jurkat Cells. Human CD28 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in Jurkat human acute T cell leukemia cell line stimulated with 10 ng/mL phorbol myristate acetate (PMA) and 0.5 μM calcium ionophore, in a dose-dependent manner, as measured using the Quantikine Human IL-2 ELISA Kit (Catalog # D2050). The ED50 for this effect is typically 0.3-0.6 μg/mL.