AF6434

antibody from Novus Biologicals

Targeting: KDM4A JHDM3A, JMJD2, JMJD2A, KIAA0677, TDRD14A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

AF6434

Provider

Novus Biologicals

Product name

Sheep Polyclonal Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.

Reactivity

Human, Mouse

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Lysine (K)-specific Demethylase 4A/KDM4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, THP-1 human acute monocytic leukemia cell line, and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Human Lysine (K)-specific Demethylase 4A/KDM4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6434) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lysine (K)-specific Demethylase 4A/KDM4A at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.