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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14135 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14135, RRID:AB_10556472
- Product name
- KDM4A polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of KDM4A.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using KDM4A polyclonal antibody (Cat # PAB14135) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of KDM4A polyclonal antibody (Cat # PAB14135), crude serum and flow through.The plates were coated with the peptide used for immunization of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 9,000.
- Validation comment
- ELISA
