A301-046A

antibody from Invitrogen Antibodies

Targeting: ARID1B 6A3-5, BAF250b, DAN15, ELD/OSA1, KIAA1235, p250R

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-046A

Provider

Invitrogen Antibodies

Product name

ARID1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody A301-046A (lot A301-046A-3) used for IP at 3 µg per reaction. ARID1B was also immunoprecipitated by a previous lot of this antibody (lot A301-046A-2) and rabbit anti-ARID1B antibody A301-047A. For blotting immunoprecipitated ARID1B, A301-046A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID1B by western blot. Samples: Whole cell lysate (15 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody A301-046A (lot A301-046A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (Cat. No. A301-046A Lot3) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB.