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LearnNB100-57485
antibody from Novus Biologicals
Targeting: ARID1B
6A3-5, BAF250b, DAN15, ELD/OSA1, KIAA1235, p250R
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [3]
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- Product number
- NB100-57485 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57485, RRID:AB_2060370
- Product name
- Rabbit Polyclonal ARID1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARID1B Antibody [NB100-57485] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20 percent of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57485 used for IP at 6 ug per reaction. For blotting immunoprecipitated ARID1B, NB100-57485 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARID1B Antibody [NB100-57485] - Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57485 (lot NB100-57485-2) used for IP at 6 ug per reaction. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-57485-1. For blotting immunoprecipitated ARID1B, NB100-57485 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57485] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-ARID1B used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ARID1B Antibody [NB100-57485] - Human ovarian carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ARID1B Antibody [NB100-57485] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID1B used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
