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LearnNB100-57484
antibody from Novus Biologicals
Targeting: ARID1B
6A3-5, BAF250b, DAN15, ELD/OSA1, KIAA1235, p250R
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-57484 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57484, RRID:AB_920648
- Product name
- Rabbit Polyclonal ARID1B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Expanding the phenotypic spectrum of ARID1B-mediated disorders and identification of altered cell-cycle dynamics due to ARID1B haploinsufficiency.
Sim JC, White SM, Fitzpatrick E, Wilson GR, Gillies G, Pope K, Mountford HS, Torring PM, McKee S, Vulto-van Silfhout AT, Jhangiani SN, Muzny DM, Leventer RJ, Delatycki MB, Amor DJ, Lockhart PJ
Orphanet journal of rare diseases 2014 Mar 27;9:43
Orphanet journal of rare diseases 2014 Mar 27;9:43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID1B Antibody [NB100-57484] - Detection of Human ARID1B on HeLa whole cell lysates using NB100-57484. ARID1B was also immunoprecipitated by rabbit anti-ARID1B antibody NB100-47485.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID1B Antibody [NB100-57484] - Detection of Human ARID1B by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody NB100-57484 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot. Samples: Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID1B antibody NB100-57484 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID1B antibody NB100-57484 (lot NB100-57484-3) used for IP at 3 ug per reaction. ARID1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-57484-2) and rabbit anti-ARID1B antibody NB100-57485. For blotting immunoprecipitated ARID1B, NB100-57484 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ARID1B Antibody [NB100-57484] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- ARID1B used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: ARID1B Antibody [NB100-57484] - Detection of human ARID1B by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ARID1B (NB100-57484). Detection: DAB
