H00001629-B01P

antibody from Abnova Corporation

Targeting: DBT BCKAD-E2, BCKDH-E2, BCOADC-E2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001629-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001629-B01P, RRID:AB_1673343

Product name

DBT purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human DBT protein.

Antigen sequence

MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPN
YVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLS
DIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDK
ASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIET
EALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATP
AVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLE
KQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPP
VFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCD
EIDLTELVKLREELKPIAFARGIKLSFMPFFLKAA
SLGLLQFPILNASVDENCQNITYKASHNIGIAMDT
EQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQ
LSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVAI
GALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVI
DGATMSRFSNLWKSYLENPAFMLLDLK


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Amino acid starvation has opposite effects on mitochondrial and cytosolic protein synthesis.

Johnson MA, Vidoni S, Durigon R, Pearce SF, Rorbach J, He J, Brea-Calvo G, Minczuk M, Reyes A, Holt IJ, Spinazzola A
PloS one 2014;9(4):e93597

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DBT expression in transfected 293T cell line (H00001629-T01) by DBT MaxPab polyclonal antibody.Lane 1: DBT transfected lysate(53.02 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DBT MaxPab polyclonal antibody. Western Blot analysis of DBT expression in human liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DBT MaxPab polyclonal antibody. Western Blot analysis of DBT expression in A-431.