34-8100
antibody from Invitrogen Antibodies
Targeting: CDC14A
cdc14, Cdc14A1, Cdc14A2, DFNB105, DFNB32
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [5]
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Validation data
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- Product number
- 34-8100 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cdc14A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion.
The CDC14A phosphatase regulates oocyte maturation in mouse.
The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.
Chen NP, Uddin B, Voit R, Schiebel E
Proceedings of the National Academy of Sciences of the United States of America 2016 Jan 26;113(4):990-5
Proceedings of the National Academy of Sciences of the United States of America 2016 Jan 26;113(4):990-5
The CDC14A phosphatase regulates oocyte maturation in mouse.
Schindler K, Schultz RM
Cell cycle (Georgetown, Tex.) 2009 Apr 1;8(7):1090-8
Cell cycle (Georgetown, Tex.) 2009 Apr 1;8(7):1090-8
The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.
Cho HP, Liu Y, Gomez M, Dunlap J, Tyers M, Wang Y
Molecular and cellular biology 2005 Jun;25(11):4541-51
Molecular and cellular biology 2005 Jun;25(11):4541-51
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cdc14A was performed using 70% confluent log phase HCT 116 cells treated with MG-132 (10 micromolar for 16h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cdc14A Rabbit Polyclonal Antibody (Product # 34-8100) at 2µg/mLin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the untreated cells with no expression of Cdc14A. Panel f shows the primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Cdc14A was done on HT-29 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Cdc14A Rabbit Polyclonal Antibody (Product # 34-8100, red histogram) or with rabbit isotype control (pink histogram) at 3-5 µg/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
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- Invitrogen Antibodies (provider)
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- Invitrogen Antibodies (provider)
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- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL