Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B11316 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B11316, RRID:AB_2687873
- Product name
- IHC-plus™ MPP5 Antibody (aa450-461) LS-B11316
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Horse, Rabbit, Simian, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation/Immunoblot Analysis:Adult rat brain lysate was immunoprecipitated overnight with 2µl of non-specific rabbit IgG (lane 1) or anti-Pals1 (lane 2) and probed with anti-Pals1 (1:1000 dilution). Arrow indicates Pals1 (~77kDa). (Data provided courtesy Dr. James Fawcett, Mt. Sinai Hospital, Toronto, Ontario)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation/Immunoblot Analysis:Adult rat brain lysate was immunoprecipitated overnight with 2µl of non-specific rabbit IgG (lane 1) or anti-Pals1 (lane 2) and probed with anti-Pals1 (1:1000 dilution). Arrow indicates Pals1 (~77kDa). (Data provided courtesy Dr. James Fawcett, Mt. Sinai Hospital, Toronto, Ontario)
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC: A 1:100 dilution of this lot stains Pals1 in confluent MDCK cells
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ICC: A 1:100 dilution of this lot stains Pals1 in confluent MDCK cells
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-MPP5 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.