LS-B11316

antibody from LSBio

Targeting: MPP5 FLJ12615, PALS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B11316

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B11316, RRID:AB_2687873

Product name

IHC-plus™ MPP5 Antibody (aa450-461) LS-B11316

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Horse, Rabbit, Simian, Xenopus

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation/Immunoblot Analysis:Adult rat brain lysate was immunoprecipitated overnight with 2µl of non-specific rabbit IgG (lane 1) or anti-Pals1 (lane 2) and probed with anti-Pals1 (1:1000 dilution). Arrow indicates Pals1 (~77kDa). (Data provided courtesy Dr. James Fawcett, Mt. Sinai Hospital, Toronto, Ontario)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation/Immunoblot Analysis:Adult rat brain lysate was immunoprecipitated overnight with 2µl of non-specific rabbit IgG (lane 1) or anti-Pals1 (lane 2) and probed with anti-Pals1 (1:1000 dilution). Arrow indicates Pals1 (~77kDa). (Data provided courtesy Dr. James Fawcett, Mt. Sinai Hospital, Toronto, Ontario)

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ICC: A 1:100 dilution of this lot stains Pals1 in confluent MDCK cells

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ICC: A 1:100 dilution of this lot stains Pals1 in confluent MDCK cells

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-MPP5 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.