AP32541PU-N

antibody from Acris Antibodies GmbH

Targeting: MPP5 FLJ12615, PALS1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32541PU-N

Provider

Acris Antibodies GmbH

Product name

anti MPP5

Antibody type

Polyclonal

Antigen

KLH-conjugated, synthetic peptide (C-YNANKNDDYDNE) corresponding to amino acids 450-461 of Human Pals1 (palmitoylated 5 protein), also known as MAGUK p55 subfamily member 5 and stardust (Drosophila).

Reactivity

Rat, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunocytochemistry: MDCK cells were grown to confluency on cover slips, washed and incubated with a 1:100 dilution of anti-Pals1 (panel A) or an antibody against the tight junction protein Zonula occludens 1 (ZO-1, Panel B). Cells were visualized using Alexa 488 secondary reagent and a fluorescent microscope. Panel C is a merged image of panels A and B. Data provided courtesy Dr. James Fawcett, Mt. Sinai Hospital, Toronto, Ontario.

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

<strong>Immmunoprecipitation/Immunoblot Analysis: </strong>Adult rat brain lysate was immunoprecipitated overnight with 2μl of non-specific rabbit IgG (lane 1) or anti-Pals1 (lane 2) and probed with anti-Pals1 (1:1000 dilution). Arrow indicates Pals1 (~77kDa). <em>Data provided courtesy Dr. James Fawcett, Mt. Sinai Hospital, Toronto, Ontario</em>