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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-28109 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MPP5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-28109 targets MPP5 in IF and WB applications and shows reactivity with Human samples. The PA5-28109 immunogen is synthetic peptide corresponding to a region within amino acids 124 and 217 of Human MPP5. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MPP5 using 30 µg of A) U87-MG and B) SK-N-SH lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MPP5 polyclonal antibody (Product # PA5-28109) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MPP5 in methanol-fixed HeLa cells using a MPP5 polyclonal antibody (Product # PA5-28109) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
