12658-1-AP

antibody from Proteintech Group

Targeting: P4HA1 C-P4Halpha(I), P4HA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12658-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12658-1-AP, RRID:AB_2283162

Product name

P4HA1 antibody

Antibody type

Polyclonal

Description

KD/KO validated P4HA1 antibody (Cat. #12658-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Redox biology 2026 Jun;93:104151

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Vascular normalization by erianin unleashes CAR-T immunotherapy in glioblastoma.

Zhou S, Qian S, Sun B, Shi P, Guo S, Yang C, Zhang J, Gong Y, Yang F
Angiogenesis 2026 Feb 12;29(2):18

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Palmitoylated SARM1 targeting P4HA1 promotes collagen deposition and myocardial fibrosis: A new target for anti-myocardial fibrosis.

Yang X, Zhang Y, Leng X, Wang Y, Gong M, Liu D, Li H, Du Z, Wang Z, Xuan L, Zhang T, Sun H, Zhang X, Liu J, Liu T, Gong T, Li Z, Liang S, Sun L, Jiao L, Yang B, Zhang Y
Acta pharmaceutica Sinica. B 2025 Sep;15(9):4789-4806

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Polydatin mitigates thrombosis by inhibiting PHD2-induced proline hydroxylation on collagen, reducing platelet adhesion.

Liu K, Cheng C, Yan J, Chi F, Wang W, Shen F, Zhang J, Zhang M, Hou Y, Bai G
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Mar;138:156392

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Protein disulfide isomerase is essential for osteoblast differentiation in mice.

Lu Y, Yang A, Zhao Z, Han Y, Wu D, Wu Y
Communications biology 2025 Mar 10;8(1):402

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Targeting P4HA1 promotes CD8(+) T cell progenitor expansion toward immune memory and systemic anti-tumor immunity.

Ma S, Ong LT, Jiang Z, Lee WC, Lee PL, Yusuf M, Ditzel HJ, Wang Y, Chen Q, Wang W, Wu X, Tan EY, Yu Q
Cancer cell 2025 Feb 10;43(2):213-231.e9

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Investigating endometrial metabolic characteristics in patients with adenomyosis using gas chromatography/mass spectrometry.

Ren J, Liu J, Dai J, Wang L, He F, Hu L
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2025 Dec;45(1):2503446

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P4HA1 is highly expressed in gastric cancer and promotes proliferation and metastasis of gastric cancer cells.

Gao P, Tian H, Ge K, Wang F, Zhao Y, Sun J, Zhang Q
Discover oncology 2025 Apr 19;16(1):575

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Dysregulated Proline Metabolism Contributes to Subretinal Fibrosis in Neovascular AMD: Therapeutic Potential of Prolyl-4-Hydroxylase Inhibition.

Zeng Y, Zhang T, Cornish E, Lee SR, Yam M, Eminhizer M, Zeng J, Zhang J, Zeng S, Wei X, Yang J, Zhu M, Chang A, Zhang M, Du J, Zhu L, Gillies MC
bioRxiv : the preprint server for biology 2025 Apr 19;

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Glycolysis-related gene signatures and the functional role of P4HA1 in osteosarcoma prognosis.

Gui H, Wang S, Li B
Experimental cell research 2025 Apr 1;447(1):114492

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Hypoxia-induced P4HA1 overexpression promotes post-ischemic angiogenesis by enhancing endothelial glycolysis through downregulating FBP1.

Xu Y, Xia D, Huang K, Liang M
Journal of translational medicine 2024 Jan 18;22(1):74

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P4HA2 hydroxylates SUFU to regulate the paracrine Hedgehog signaling and promote B-cell lymphoma progression.

Li Q, Liu Y, Wu J, Zhu Z, Fan J, Zhai L, Wang Z, Du G, Zhang L, Hu J, Ma DK, Liu JO, Huang H, Tan M, Dang Y, Jiang W
Leukemia 2024 Aug;38(8):1751-1763

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Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma.

Sun Y, Chen Y, Zhao H, Wang J, Liu Y, Bai J, Hu C, Shang Z
iScience 2024 Apr 19;27(4):109340

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P4HA1 activates HMGCS1 to promote nasopharyngeal carcinoma ferroptosis resistance and progression.

Zhou R, Qiu L, Zhou L, Geng R, Yang S, Wu J
Cellular signalling 2023 May;105:110609

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Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation.

Chen G, Tang Q, Yu S, Shen Y, Sun J, Peng J, Yin Y, Feng G, Lu X, Mei G, Zhang Y, Wan Q, Zhang L, Chen L
Cell death and differentiation 2023 Jun;30(6):1503-1516

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Exploring the glycolytic cross-talk genes between inflammatory bowel disease and colorectal cancer.

Ye C, Huang Y, Gao Y, Zhu S, Yuan J
Functional & integrative genomics 2023 Jul 10;23(3):230

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P4HA1 expression and function in esophageal squamous cell carcinoma.

Gou W, Song B, Yang Y
Medicine 2023 Dec 22;102(51):e36800

---

P4HA1, transcriptionally activated by STAT1, promotes esophageal cancer progression.

Gou W, Yang Y, Shan Q, Xia S, Ma Y
Pathology international 2023 Apr;73(4):147-158

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IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion.

Chen X, Zhao J, Herjan T, Hong L, Liao Y, Liu C, Vasu K, Wang H, Thompson A, Fox PL, Gastman BR, Li X, Li X
The Journal of experimental medicine 2022 Jun 6;219(6)

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P4HA1 Down-Regulation Inhibits Glioma Invasiveness by Promoting M1 Microglia Polarization.

Wang Q, Zhang J, Fang S, Wang J, Han X, Liu F, Jin G
OncoTargets and therapy 2021;14:1771-1782

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Overexpression of P4HA1 associates with poor prognosis and promotes cell proliferation and metastasis of lung adenocarcinoma.

Ning Y, Zheng H, Zhan Y, Liu S, Yang Y, Zang H, Wen Q, Zhang Y, Fan S
Journal of Cancer 2021;12(22):6685-6694

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P4HA1 as an unfavorable prognostic marker promotes cell migration and invasion of glioblastoma via inducing EMT process under hypoxia microenvironment.

Zhu X, Liu S, Yang X, Wang W, Shao W, Ji T
American journal of cancer research 2021;11(2):590-617

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Identification of a novel glycolysis-related gene signature for predicting the prognosis of osteosarcoma patients.

Yang M, Ma X, Wang Z, Zhang T, Hua Y, Cai Z
Aging 2021 May 5;13(9):12896-12918

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Cotranslational prolyl hydroxylation is essential for flavivirus biogenesis.

Aviner R, Li KH, Frydman J, Andino R
Nature 2021 Aug;596(7873):558-564

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HIF-1α interacts with Kindlin-2 and influences breast cancer elasticity: A study based on shear wave elastography imaging.

Xue X, Xue S, Wan W, Li J, Shi H
Cancer medicine 2020 Jul;9(14):4971-4979

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Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.

Eriksson J, Le Joncour V, Jahkola T, Juteau S, Laakkonen P, Saksela O, Hölttä E
Molecular oncology 2020 Apr;14(4):742-762

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MiR-122 inhibits epithelial mesenchymal transition by regulating P4HA1 in ovarian cancer cells.

Duan Y, Dong Y, Dang R, Hu Z, Yang Y, Hu Y, Cheng J
Cell biology international 2018 Nov;42(11):1564-1574

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P4HA1 mutations cause a unique congenital disorder of connective tissue involving tendon, bone, muscle and the eye.

Zou Y, Donkervoort S, Salo AM, Foley AR, Barnes AM, Hu Y, Makareeva E, Leach ME, Mohassel P, Dastgir J, Deardorff MA, Cohn RD, DiNonno WO, Malfait F, Lek M, Leikin S, Marini JC, Myllyharju J, Bönnemann CG
Human molecular genetics 2017 Jun 15;26(12):2207-2217

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MiR-30e suppresses proliferation of hepatoma cells via targeting prolyl 4-hydroxylase subunit alpha-1 (P4HA1) mRNA.

Feng G, Shi H, Li J, Yang Z, Fang R, Ye L, Zhang W, Zhang X
Biochemical and biophysical research communications 2016 Apr 8;472(3):516-22

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Extracellular matrix remodeling by bone marrow fibroblast-like cells correlates with disease progression in multiple myeloma.

Slany A, Haudek-Prinz V, Meshcheryakova A, Bileck A, Lamm W, Zielinski C, Gerner C, Drach J
Journal of proteome research 2014 Feb 7;13(2):844-54

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The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.

Chakravarthi BV, Pathi SS, Goswami MT, Cieślik M, Zheng H, Nallasivam S, Arekapudi SR, Jing X, Siddiqui J, Athanikar J, Carskadon SL, Lonigro RJ, Kunju LP, Chinnaiyan AM, Palanisamy N, Varambally S
Oncotarget 2014 Aug 30;5(16):6654-69

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The effect of isosaponarin isolated from wasabi leaf on collagen synthesis in human fibroblasts and its underlying mechanism.

Nagai M, Akita K, Yamada K, Okunishi I
Journal of natural medicines 2010 Jul;64(3):305-12

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The reverse Warburg effect: aerobic glycolysis in cancer associated fibroblasts and the tumor stroma.

Pavlides S, Whitaker-Menezes D, Castello-Cros R, Flomenberg N, Witkiewicz AK, Frank PG, Casimiro MC, Wang C, Fortina P, Addya S, Pestell RG, Martinez-Outschoorn UE, Sotgia F, Lisanti MP
Cell cycle (Georgetown, Tex.) 2009 Dec;8(23):3984-4001

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 12658-1-AP(P4HA1 antibody) at dilution of 1:600

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-P4HA1 (IP:12658-1-AP, 3ug; Detection:12658-1-AP 1:600) with A431 cells lysate 3300ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The P4HA1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human P4HA1. This antibody recognizes human,mouse,rat antigen. The P4HA1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.

Appendix

12658-1-AP.pdf

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