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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-52454 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P4HA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FTSIGQMTDL IHTEKDLVTS LKDYIKAEED KLEQIKKWAE KLDRLTSTAT KDPEGFVGHP VNAFKLMKRL NTEWSELENL VLKDMSDGFI SNLTIQRQYF PNDEDQVGAA KALLRLQDTY NLDTDTISKG NLPGVKHKSF LTAEDC
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P4HA1 using P4HA1 Polyclonal Antibody (Product # PA5-52454) (A) shows similar pattern to an independent P4HA1 Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of P4HA1 in human cell line U-251 MG shows positivity in endoplasmic reticulum. Samples were probed using a P4HA1 Polyclonal Antibody (Product # PA5-52454).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA1 in human testis using a P4HA1 Polyclonal Antibody (Product # PA5-52454) shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA1 in human pancreas using P4HA1 Polyclonal Antibody (Product # PA5-52454) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of P4HA1 in human placenta using P4HA1 Polyclonal Antibody (Product # PA5-52454) shows high expression.
