PA5-52454

antibody from Invitrogen Antibodies

Targeting: P4HA1 C-P4Halpha(I), P4HA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52454

Provider

Invitrogen Antibodies

Product name

P4HA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FTSIGQMTDL IHTEKDLVTS LKDYIKAEED KLEQIKKWAE KLDRLTSTAT KDPEGFVGHP VNAFKLMKRL NTEWSELENL VLKDMSDGFI SNLTIQRQYF PNDEDQVGAA KALLRLQDTY NLDTDTISKG NLPGVKHKSF LTAEDC Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.18 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of P4HA1 using P4HA1 Polyclonal Antibody (Product # PA5-52454) (A) shows similar pattern to an independent P4HA1 Polyclonal Antibody (B).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of P4HA1 in human cell line U-251 MG shows positivity in endoplasmic reticulum. Samples were probed using a P4HA1 Polyclonal Antibody (Product # PA5-52454).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of P4HA1 in human testis using a P4HA1 Polyclonal Antibody (Product # PA5-52454) shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of P4HA1 in human pancreas using P4HA1 Polyclonal Antibody (Product # PA5-52454) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of P4HA1 in human placenta using P4HA1 Polyclonal Antibody (Product # PA5-52454) shows high expression.