Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [8]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-84398 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84398, RRID:AB_11015709
- Product name
- Rabbit Polyclonal P4HA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human P4HA1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.
Eriksson J, Le Joncour V, Jahkola T, Juteau S, Laakkonen P, Saksela O, Hölttä E
Molecular oncology 2020 Apr;14(4):742-762
Molecular oncology 2020 Apr;14(4):742-762
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: P4HA1 Antibody [NBP1-84398] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: P4HA1 Antibody [NBP1-84398] - Analysis in human cell lines PC-3 and HEK293 using Anti-P4HA1 antibody. Corresponding P4HA1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: P4HA1 Antibody [NBP1-84398] - Analysis using Anti-P4HA1 antibody NBP1-84398 (A) shows similar pattern to independent antibody NBP1-84397 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human placenta shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human cerebellum shows moderate granular cytoplasmic positivity in Purkinje cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human endometrium shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human placenta shows strong cytoplasmic positivity in decidual cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: P4HA1 Antibody [NBP1-84398] - Staining in human placenta and pancreas tissues using NBP1-84398 antibody. Corresponding P4HA1 RNA-seq data are presented for the same tissues.