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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- TA505753 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505753, RRID:AB_2623344
- Product name
- BBOX1 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)
- Antibody type
- Monoclonal
- Description
- BBOX1 mouse monoclonal antibody, clone OTI4F9 (formerly 4F9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BBOX1
- Isotype
- IgG
- Antibody clone number
- OTI4F9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1 (RC203156, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BBOX1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-BBOX1 mouse monoclonal antibody (TA505753) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1(RC203156).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-BBOX1 mouse monoclonal antibody (TA505753).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505753)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505753)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BBOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505753)
- Validation comment
- IHC
