PA5-21477

antibody from Invitrogen Antibodies

Targeting: BBOX1 BBH, BBOX, G-BBH, gamma-BBH

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21477

Provider

Invitrogen Antibodies

Product name

BBOX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Molt-4, mouse liver. Predicted reactivity: Mouse (86%), Rat (85%), Bovine (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.76 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae.

Jiang D, Burger CA, Casasent AK, Albrecht NE, Li F, Samuel MA
The Journal of comparative neurology 2020 Apr 1;528(5):729-755

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Gamma-butyrobetaine dioxygenase in methanol-fixed A431 cells using a Gamma-butyrobetaine dioxygenase polyclonal antibody (Product # PA5-21477) at a 1:500 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A431, using BBOX1 antibody (Product # PA5-21477) at 1:500 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Bbox1 labels the ganglion cell layer (GCL) in adult retina. (a,b) Immunohistochemical images of Bbox1 (green) at lower (a) and higher (b) magnification in 14-week-old retina reveal that Bbox1 is exclusively localized to the GCL. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. (c) Representative images showing the major cell classes residing in the adult GCL: RGCs (RBPMS), astrocytes (GFAP), and displaced starburst amacrines (ChAT; white arrows). (d) Quantification of the major cell classes in the GCL in adult 14-week-old retina (RBPMS, retinal ganglion cells; GFAP, astrocytes; ChAT, displaced amacrine cells). n = 3 animals. (e,f) Representative images (e) and quantification (f) of Bbox1 (green) and astrocytes (magenta) colocalization in adult animals. Bbox1 shows very little overlap with astrocytes, which reside below GCL neurons. (g,h) Representative images (g) and quantification (h) of Bbox1 (green) and ChAT amacrine costaining in adult retina. Bbox1 does not label ChAT amacrines in the INL (yellow asterisks) but does label displaced ChAT amacrines in the GCL (white arrows). (i,j) Representative images (i) and quantification (j) of Bbox1 (green) and ganglion cells (RBPMS, magenta) colocalization in adult retina. Bbox1 labels all ganglion cells in the GCL and also labels displaced ganglion cells in the INL (white arrows). n = 3 animals. Data are represented as the mean +- SEM . Scale bars = 50 mum [Color figure can