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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00004632-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004632-M01, RRID:AB_2250966
- Product name
- Mouse Monoclonal Fast skeletal myosin light chain 1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MYL1 (2D9)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Analysis of MYL1 over-expressed 293 cell line, cotransfected with MYL1 Validated Chimera RNAi ( Cat # H00004632-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYL1 monoclonal antibody (M01), clone 2D9 (Cat # H00004632-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Analysis of MYL1 expression in transfected 293T cell line by MYL1 monoclonal antibody (M01), clone 2D9.Lane 1: MYL1 transfected lysate(21.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Detection limit for recombinant GST tagged MYL1 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fast skeletal myosin light chain 1 Antibody (2D9) [H00004632-M01] - Analysis of monoclonal antibody to MYL1 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.
