PA5-29635

antibody from Invitrogen Antibodies

Targeting: MYL1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29635

Provider

Invitrogen Antibodies

Product name

MYL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, mouse heart, rat muscle. Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (83%), Pig (94%), Rabbit (93%), Chicken (82%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.28 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy.

Li L, Jing L, Zhao J, Lv J, Yang W, Li W, Zhou L
Experimental and therapeutic medicine 2019 Dec;18(6):4313-4321

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Fast skeletal myosin light chain 1 in paraformaldehyde-fixed HeLa cells using a Fast skeletal myosin light chain 1 polyclonal antibody (Product # PA5-29635) at a 1:500 dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Effect of valsartan on protein expression level of RhoA, ROCK-2 and MYL in myocardial cells-amelioration by valsartan. (A) Western blots results of RhoA, ROCK-2 and MYL expression in myocardial cells. The experiment was repeated 3 times with similar results. The figure presents a representative analysis. Quantification of the western blots (B) RhoA, (C) ROCK-2 (D) and MYL. Columns are means +/- standard error of the mean, (n=3). *P